Using a camera to take horizontal root pictures

Guillaume Lobet | 2012-06-17 | root image camera

One way to take root picture using a camera is to put the root system on a flat surface and take picture from above. This technique is fast and can be used with large root system. Here is a couple of things to think of before taking such image. Choose the proper camera and lens (A) Always place the camera at the same distance of the roots (B) Use a dark, matt background (C) Place a scale on the background (D) Carefully separate the different roots About the root system This technique can be… More...

Acquiring root images

Guillaume Lobet | 2012-06-16 | root image analysis

The image acquisition step is a critical one as it will determine the quantity of information contained in the final image. Fortunately, good images can be obtain fairly simply without buying fancy expensive material. In the next posts I will give simple tips to acquire nice root images with either a CCD camera or a scanner (which are probably the most widely used image acquisition devices as well as the less expensive). But first a small reminder on the image requirements. Image requirement In… More...

Cleaning root systems

Guillaume Lobet | 2012-06-16 | root

If you want to take a picture or scan a root system, one of the requirements is that this root system is clean of soil. While this is not an issue if the plant is grown in aeroponic or hydroponics, it is important for field- / pot-grown plants.The easiest way to clean roots is to simply let them soak in water, eventually with soap for several minutes. Then take them out and use pressurised water to remove the mud.If you need to clean smaller root or finer particles, you can use a brush to… More...

How to choose the right root image analysis tool

Guillaume Lobet | 2011-12-06 | root image analysis

In this post, I will try to point out the most important characteristics you will have to consider while choosing your root image analysis software. Very often, there is a strong link between an experimental design and the software used to treat the root images. Be careful that you should not be designing your experiment according to the available software in your lab, but the other way round. As there is more and more available software, the field of possible analysis widens. Do not hesitate… More...

What makes a root image suitable for analysis?

Guillaume Lobet | 2011-06-11 | root image analysis

The quality of an image is central if you want to get useful information out of it. Even the best software will not be able to analyse crappy images, so it is crucial to think about what makes a good image and how to acquire it. Usually, you will have the opportunity to improve your images at different steps of the process: the growth of the plant (substrate, ...) the image acquisition (the type of devise) the image preparation (thresholding,.. ) the image analysis (the type of measurement,… More...
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