Thresholding root images

Guillaume Lobet | 2010-12-15 | root image analysis thresholding

Many root image analysis procedures have a thresholding step in their workflow (e.i. WinRHIZO or EZ-RHIZO). The thresholding step aims at segmenting the original image (fig. 1A) in two parts: the "object" (the roots) and the "background", and to assign to each of them a different pixel value (typically 1 and 0). The subsequent binary image (fig. 1B) can be used, for instance, to generate a "root skeleton" (fig. 1C) or to estimate root diameter. Figure 1. A.… More...
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